Epac1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90465-50, BS90465-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O95398(Human) Q8VCC8(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC, IP, ICC/IF

All Applications: WB:1:500-1:1,000
IHC:1:50-1:200
IP:1:10-1:50
ICC/IF:1:10-1:50

Background: 3',5' cyclic adenosine monophosphate (cAMP)-regulated guanine nucleotide exchange factors Epac (Epac1, cAMP-GEFI) and Epac2 (cAMP-GEFII) activate the Ras family GTPases Rap 1 and Rap 2 by promoting GTP binding in a cAMP-dependent manner. Eukaryotic cAMP is a second messenger that induces physiological responses such as gene expression, growth, differentiation, secretion and neurotransmission. The human Epac gene maps to chromosome 12q13.11 with transcript being abundant in the kidney and heart. In situ hybridization indicates expression of Epac in adult rat brain and selective expression in neonatal brain, including septum and thalamus.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Epac1 polyclonal antibody detects endogenous levels of Epac1 protein.

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Epac1 on mouse kidney tissue lysate using anti-Epac1 antibody at 1/1,000 dilution., Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Epac1 antibody. Counter stained with hematoxylin.