Dulbecco’s Modified Eagle Medium is the most commonly used modification of the Basal Medium acc. to Eagle. DMEM contains higher concentration of amino acids and vitamins, and additionally iron (ferric nitrate). The basic composition of ROTI^®Cell DMEM media corresponds to the original formulation according to Dulbecco.The formulation with low glucose content is well suited for culturing of a variety of mammalian adherent cell lines.

Generally, FBS is added to DMEM basal media.

This modification of DMEM contains pyruvate, but no glutamine.