Recombinant Human D-Amino-Acid Oxidase/DAO Protein (His Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH032342-10, PKSH032342-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: DAO

Target Synonym: D-Amino-Acid Oxidase; DAAO; DAMOX; DAO

UNIProt ID: P14920

Research Areas: Signal Transduction; Tags & Cell Markers; metabolism;

Target Species: Human

Expression Host: E.coli

Fusion Tag: N-6His

Accession: P14920

Background: D-Amino-Acid Oxidase (DAO) belongs to the DAMOX/DASOX family. DAO is a peroxisomal enzyme which founctions as a homodimer to oxidizes D-amino acids to the corresponding imino acids, producing ammonia and hydrogen peroxide. D-amino-acid oxidase regulates the level of the neuromodulator D-serine in the brain, has a high activity towards D-DOPA and contributes to dopamine synthesis. D-amino-acid oxidase could act as a detoxifying agent which removes D-amino acids accumulated during aging. It also acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups.

Sequence: Met 1-Leu347

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM DTT, 2mM EDTA, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 41.6 kDa

Observed MW: 40 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only