RXRA Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91189-50, BS91189-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P19793(Human) P28700(Mouse) Q05343(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IF, IHC, IP,

All Applications: WB:1:1,000-1:5,000
IHC:1:50-1:200
ICC:1:50-1:200
IP:1:10-1:50

Background: Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: RXRA polyclonal antibody detects endogenous levels of RXRA protein.

Bioworld Molecular Weight: 51 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Retinoid X Receptor alpha on MCF-7 cell using anti-Retinoid X Receptor alpha antibody at 1/5,000 dilution., ICC staining Retinoid X Receptor alpha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: RXRA, NR2B1, FLJ00280, FLJ00318, FLJ16020, FLJ16733, MGC102720, NR2B1, Nuclear receptor subfamily 2 group B member 1, OTTHUMP00000022510, Retinoic acid receptor RXR alpha, Retinoic acid receptor RXR-alpha, Retinoid X nuclear receptor alpha, Retinoid X receptor alpha, RXR alpha1, Rxra, RXRA, RXRalpha1,

Immunogen: Recombinant protein corresponding to N-terminal human Retinoid X Receptor alpha.

Conjugate: Unconjugated

Modification: Unmodified