Human CCK18 (Cholecystokinin 18) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK4875-48, ELK4875-96, ELK4875-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: CCK18

Assay Type: Competitive Inhibition

Sensitivity: 4.28 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Endocrinology; Gastroenterology; Hormone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CCK18. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CCK18. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CCK18 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only