ATG3 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB10585-50, MB10585-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: Q9NT62

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Background: E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). Catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 36 kDa; Observed MW: 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of ATG3 in K562, rat Brain, C6, 3T3, Hela lysates using ATG3 antibody., Immunocytochemistry analysis of ATG3 in Hela using ATG3 antibody, and DAPI, Immunohistochemistry analysis of paraffin-embedded Human tonsil using ATG3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: APG3; APG3L; PC3-96; APG3-LIKE

Immunogen: A synthetic peptide of human ATG3

Conjugate: Unconjugated

Modification: Unmodified