HMGB3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS76103-50, BS76103-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O15347

Host: Rabbit

Reactivity: Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene encodes a member of a family of proteins containing one or more high mobility group DNA-binding motifs. The encoded protein plays an important role in maintaining stem cell populations, and may be aberrantly expressed in tumor cells. A mutation in this gene was associated with microphthalmia, syndromic 13. There are numerous pseudogenes of this gene on multiple chromosomes. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of Rat thymus, using HMGB3 antibody at 1:2265 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.

Note: For research use only, not for use in diagnostic procedure.

Bioworld Molecular Weight: 25KDa

Alternative Name: HMGB3; HMG-2a; HMG-4; HMG2A; HMG4

Immunogen: A synthetic peptide of human HMGB3(NP_005333.2).

Conjugate: Unconjugated

Modification: Unmodified