STAT3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91286-50, BS91286-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P40763(Human) P42227(Mouse) P52631(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC

All Applications: WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200

Background: Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by Prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: STAT3 polyclonal antibody detects endogenous levels of STAT3 protein.

Bioworld Molecular Weight: 88 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of STAT3 on MCF-7 cells lysates using anti-STAT3 antibody at 1/1,000 dilution., ICC staining STAT3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: STAT3, 1110034C02Rik, Acute Phase Response Factor, Acute-phase response factor, ADMIO, APRF, AW109958, DNA binding protein APRF, FLJ20882, HIES, MGC16063, Signal transducer and activator of transcription 3 (acute phase response factor), Signal transducer and activator of transcription 3, STAT 3, Stat3, STAT3,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified