Smad3 (4H4) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB3333-50, MB3333-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P84022

Host: Mouse

Reactivity: Human, Rat, Mouse

Applications: WB, IHC-P

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100

Background: Smad3 transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG1

Bioworld Molecular Weight: Calculated MW: 48 kDa; Observed MW: 48 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Smad3 in 293T, Jurkat, rat Heart, mouse Heart lysates using Smad3 antibody., Immunohistochemistry analysis of paraffin-embedded Human Liver Tissue using Smad3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunohistochemistry analysis of paraffin-embedded Human Colon Carcinoma using Smad3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

Immunogen: Purified recombinant protein expressed in E.coli.

Conjugate: Unconjugated

Modification: Unmodified